Clear Choices for qPCR and qRT-PCR

USB^® HotStart-IT^® qPCR: Select from SYBR, Probe-based master mixes with & without UDG Carry Over Prevention 

HotStart-IT^® SYBR^® Green One-Step qRT-PCR Master Mix Kit

Benefits

• Detect very low levels of target RNA, fewer than 10 copies
• Start with as little as 1 pg of total RNA or 100 fg of mRNA 
• Universal master mix with SYBR^® Green -- No special probes needed for amplicon detection
• Uses standard SYBR filters (typically FAM setting)
• Performance over a linear dynamic range of 7 to 8 orders of magnitude with a minimal correlation coefficient ³0.95

HotStart-IT^® Probe One-Step qRT-PCR Master Mix Kit

Benefits

• Detect very low levels of target RNA, fewer than 10 copies
• Start with as little as 1 pg of total RNA or 100 fg of mRNA 
• Universal master mix with fluorescent probe detection
• Compatible with TaqMan^® probes, Molecular Beacons, etc.
• Performance over a linear dynamic range of 7 to 8 orders of magnitude with a minimal correlation coefficient ³0.95

HotStart-IT^® SYBR^® Green qPCR Master Mix

Benefits

• All-in-one universal master mix with SYBR^® Green
  • Compatible with standard SYBR filters
  • Multiple platform capability - includes separate tubes of ROX™ and Fluorescein Passive Reference Dyes to allow for normalization on different instruments
• Highest sensitivity with broad dynamic range
  • Detects fewer than 10 target copies
  • Performs over a linear dynamic range of 7 to 8 orders of magnitude with a minimal correlation coefficient ³0.95

HotStart-IT^® Probe qPCR Master Mix

Benefits

• All-in-one universal master mix for use with fluorescent probes
  • Compatible with TaqMan^® probes, Molecular Beacons, FRET probe pairs, etc.
  • Multiple platform capability - includes ROX™ Reference Dye to allow normalization of well-to-well variations
• Highest sensitivity with broad dynamic range
  • Detects fewer than 10 target copies
  • Performs over a linear dynamic range of 7 to 8 orders of magnitude with a minimal correlation coefficient ³0.95

HotStart-IT^® qPCR Master Mixes with UDG

Benefits

• Effective Carry Over Contamination Prevention – Eliminate at least 10⁵ copies of dUTP-containing contaminating templates
• Heat-labile UDG is included in master mixes
  • UDG is completely and irreversibly heat-inactivated, unlike E.coli UDG
  • Optimized dUTP to dTTP ratio for enhanced sensitivity
• All-in-one universal master mixes – May be used on multiple instruments
• Flexible – Choose either SYBR^®-based or probe-based master mixes