RapidRun™ Agarose Buffer, 20X Solution

Applications:

1. High voltage, fast agarose gel electrophoresis (20-30 volts/cm).
2. This buffer is compatible with commercial gel extraction kits. However, the pH should be adjusted with sodium acetate, pH 5.0, as recommended by the manufacturer.

Description:
RapidRun Agarose Buffer allows for gels to be run in one-fourth the time required for standard buffers, such as TAE and TBE. The buffer is a non-borate weak acid that enables high voltage and more rapid electrophoretic runs (Fig 1). Temperature seldom exceeds 38°C during high voltage runs, minimizing the band diffusion and gel degradation commonly associated with TAE and TBE under the same high voltage conditions.

20X RapidRun Agarose Buffer consists of 1.78M Tris, 0.57M Taurine, and 0.01M EDTA. The solution is 0.2 μm filtered.
 
Note: 1 ml of RapidRun Loading Dye (PN 77524) is included with each pack size. The dye front migrates lower than 50 bases.

Contaminant Testing:
DNase (endo): Passes test
DNase (exo): Passes test
RNase: Passes test

pH (1:20, 25°C): 8.9 + 0.1

Conductivity (1X):
830 + 100 µmhos/cm

Testing:
Meets or exceeds functional test criteria for high voltage (300v, 15 min) mini-agarose gel electrophoresis.

Storage:
Ambient. Crystals which form at low temperatures can be re-dissolved by warming briefly.