Ligate-IT™ Rapid Ligation Kit

Description:
Ligate-IT™ Rapid Ligation Kit provides high-quality reagents for fast and easy ligation reactions.

Quick and Efficient
Ligation reactions are complete following room temperature incubation in just 5 minutes for cohesive-ends and 10 minutes for blunt-ends (see Figs. 1-2). Transform competent cells immediately or store reactions at -20°C for later transformation.

Convenient
Ligate-IT Rapid Ligation Kit includes USB T4 DNA Ligase, Nuclease-Free Water, and a specially formulated 5X Reaction Buffer which enhances the activity of T4 DNA Ligase, especially on blunt-end fragments. All of the components are in a ready-to-use format and do not require any additional reagents, such as ATP.

Flexible
The kit can be used for a variety of ligation procedures such as conventional vector cloning, TA cloning, linker or adaptor ligation, and library construction.

Kit Components                                       25 Reaction      100 Reaction
                                                                   Pack Size          Pack Size
Ligate-IT T4 DNA Ligase (PN 78401)                  25 µl                  100 µl
5X Ligate-IT Reaction Buffer (PN 78402)             100 µl                400 µl
Water, Nuclease-Free (PN 71786)                     1 ml                   2 x 1 ml

Recombinant T4 DNA Ligase is supplied in 25mM Tris-HCl (pH 7.6), 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol. Kit shipped on dry ice. Store components at -20°C in a non-frost-free freezer.

Tested User Friendly^TM Functional Test:
Cohesive- and blunt-end ligations of a 1200 bp insert into pUC19 yielded 1 x 10⁶ transformants per microgram of linearized, dephosphorylated vector.

References:
1. SAMBROOK, J. AND RUSSELL, D. W. (2001) “Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory Press, 1.84-1.125.