Protein Markers, 10 - 225 kDa

Description:
The 10-225 kDa Protein Markers from USB are recombinant proteins designed for convenient and accurate sizing of protein samples. Unlike conventional protein markers, the recombinant marker proteins do not contain oligosaccharides which often cause poor resolution and inaccurate size estimation of protein bands.

An added benefit is the ability to estimate the concentration of protein samples using the known mass for each protein band. Each vial (500 µl) contains 500 µg of protein - 50 µg per band except for 100 µg of the 50 kDa high intensity reference band.

The markers are in ready-to-load form in gel loading buffer. Simply add 5 µl of the marker to a standard SDS-polyacrylamide gel for visualization with Coomassie® blue.

As an added convenience, 2X SDS Sample Buffer is included for preparing protein samples for gel electrophoresis.

Protein Bands (MW): 10 kDa; 15 kDa; 25 kDa; 35 kDa; 50 kDa; 75 kDa; 100 kDa; 150 kDa; 225 kDa. The 50 kDa band is a high intensity reference band.

Storage Buffer:
1 µg/µl total marker protein in 125mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 200mM β-ME, 0.007% bromophenol blue.

Functional Test:
Meets or exceeds criteria for SDS-PAGE.

To Use: Add 5 µl of marker per gel lane on standard SDS-polyacrylamide gels for staining with Coomassie blue. For silver staining or S-Taq analysis, use 5 µl of a 1:25 dilution in 1X Sample Buffer.

2X SDS Sample Buffer (PN 76745, 1 ml  included with 76740):
Formulation: 250mM Tris-HCl, pH 6.8, 6% SDS, 300mM DTT, 30% glycerol and 0.02% bromophenol blue.Shipped on ice. Store at +4°C. Alternatively, store at -20°C in working aliquots.

Storage:
Shipped on ice. Store at 4°C.